[Expired] PhD thesis on a novel mechanism of bacterial copper resistance

The objective of the PhD thesis is to describe an original Cu resistance mechanism of environmental and pathogenic bacteria. This mechanism occurs under anaerobiosis or micro-aerobiosis and involves the periplasmic cuproprotein CopI. We will study the CopI proteins from three models: the environmental bacterium Rubrivivax gelatinosus and the pathogenic bacteria Vibrio cholerae and Pseudomonas aeruginosa. We will produce and purify the WT proteins as well as several mutants. The different Cu sites will be fully characterized, in particular by state-of-the-art magnetic resonance spectroscopy (EPR and NMR). Redox properties and electron transfer pathways will be studied. Two potential partner proteins, CopJ and CopH, selected from genome analysis, will also be studied.

Web: https://bip.cnrs.fr/groups/bip07/