1st FrenchBIC summer school: from september 17th to 21st, 2017

 Cette école permettra à une quarantaine de jeunes chercheurs/chercheuses (du doctorat aux premières années après le recrutement chercheur·e/ingénieur·e) de se former grâce à des cours et des travaux pratiques aux méthodes d’études des métaux en biologie. Le petit nombre de participant·e·s permettra d’organiser des TPs en petits groupes et de favoriser les interactions avec les enseignant·e·s. Les inscriptions seront bon marché pour favoriser la participation des plus jeunes. Les personnes employées par le CNRS peuvent obtenir de la formation permanente la prise en charge complète de leurs frais. Pour participer à cette école, la première étape consiste à remplir le formulaire ci dessous. Le comité d’organisation sélectionnera les participant·e·s qui devront ensuite s’inscrire.

  This summer school will provide training for young researchers, young assistant professors, engineers and technicians, PhD students and post doctoral researchers. About 45 people will attend. This number is small, in order to favour the interactions between the participants and the teachers (who will be staying for the entire duration of the school), and to run practical sessions in small groups of students. The techniques that are most useful for studying metals in biology will introduced, including traditional methods (EPR, electrochemistry, Mössbauer, NMR, RMN, fast kinetics) and a presentation of modern methods (X fluorescence imaging, mass spectrometry, nanoSIMS, AFM), which allow us to study metals in their complex biological environments.

The generosity of our sponsors has allowed us to decrease the registration fees to 150€ (this includes accommodation, meals and transportation between Carry le Rouet and Marseille). Further reduction applies if you are a member of the Société Française de Chimie.  You are entitled free registration and travel if you are paid by CNRS (irrespective of whether you are permanent researcher, technical staff, PhD student or postdoctoral researcher).

To attend, the first step consists in applying using the form below. The organization committee will then select the successful candidates and let them know. In a second step, the selected participants will have to register and pay for this, unless their fees are covered by the CNRS.

Seven lectures (two hours each)

• L1. EPR. Bruno Guigliarelli, AMU/CNRS, Marseille.
  Download the slides (pdf).
• L2. NMR. Françoise Guerlesquin, CNRS/AMU, Marseille.
• L3. Theoretical chemistry, Jean-Marie Mouesca, CEA/Univ Grenoble Alpes.
  Download the slides (pdf).
• L4. High resolution chemical imaging of metal ions in biology: nanoSIMS, X-Ray fluorescence and transmission electron microscopy. Sergio Marco, Institut Curie / INSERM, Paris. Download the slides (pdf).
• L5. Metal cations in intricate biological media. Clotilde Policar, ENS, Paris.
• L6. Mössbauer Spectroscopy. Geneviève Blondin, CNRS/CEA Grenoble.
• L7. Mass spectrometry as a analytical tool for metal complexes in biological samples, Joelle Vinh, ESPCI/CNRS, Paris.

Four practicals (half a day each)

• NMR, Françoise Guerlesquin & Olivier Bornet, CNRS/AMU, Marseille. (Files (zip)).
• EPR, Emilien Etienne, Marlène Martinho, Bénédicte Burlat et Bruno Guigliarelli, CNRS/AMU, Marseille.
• Electrochemistry. Vincent Fourmond & Christophe Léger, CNRS/AMU, Marseille.
• One to choose in this list: (*)
  • High resolution chemical imaging of metal ions in biology: image analysis. Sergio Marco, Institut Curie / INSERM, Paris.
  • DFT, Maylis Orio, CNRS/AMU, Marseille.
  • Stopped flow, Jalila Simaan, CNRS/AMU, Marseille.
  • SQUID, Sylvain Bertaina, CNRS/AMU, Marseille.

(*) The organization committee will try to follow the applicants’ requests.

• EPR
  • (en français) La spectroscopie de Résonance Paramagnétique Electronique, volume 1 Patrick Bertrand, EDP Sciences, collection Grenoble Sciences, (2010).
  • “Metallomic EPR spectroscopy,” Hagen WR, Metallomics 1 384-91(2009) . doi: 10.1039/b907919j
  • “EPR spectroscopy as a probe of metal centres in biological systems.” Hagen WR, Dalton Trans. 37, 4415-34  (2006) doi: 10.1039/b608163k
  • “Principles and applications of EPR spectroscopy in the chemical sciences” Maxie M. Roessler and Enrico Salvadori, Chemical Society Reviews, 2018, 10.1039/C6CS00565A
• NMR
  • “NMR Spectroscopy of Paramagnetic Metalloproteins”, Ivano Bertini et al, ChemBioChem, 6 1536, (2005) DOI: 10.1002/cbic.200500124
  • “An Introduction to Biological NMR Spectroscopy”, Dominique Marion, Molecular & Cellular Proteomics 12, 3006, (2013) doi: 10.1074/mcp.O113.030239 (open access)
• Electrochemistry
  • “Protein Electrochemistry: Questions and Answers” V. Fourmond and C. Leger, Adv Biochem Eng Biotechnol. (2016) doi: 10.1007/10_2015_5016
  • “Direct electrochemistry of redox enzymes as a tool for mechanistic studies”C. Leger and P. Bertrand. , Chem. Rev. 108, 2379 (2008) doi:10.1021/cr0680742 (open access)
• Mössbauer
  • “Mössbauer spectroscopy of Fe/S proteins,” Pandelia et al,  Biochim Biophys Acta.  1853, 1395 (2015). doi: 10.1016/j.bbamcr.2014.12.005
• DFT
  • “A critical evaluation of DFT, including time-dependent DFT, applied to bioinorganic chemistry,” F Neese, J Biol Inorg Chem (2006) 11:702–711 DOI 10.1007/s00775-006-0138-1
  • “Prediction of molecular properties and molecular spectroscopy with density functional theory: From fundamental theory to exchange-coupling,” Frank Neese Coord. Chem. Rev. (2008)
    doi: 10.1016/j.ccr.2008.05.014
  • “Dealing with complexity in open-shell transition metal chemistry from a theoretical perspective,” F Neese et al, Th. & Comput. Inorg. Chem. (2010) Elsevier Inc. VOLUME 62 ISSN 0898-8838 / doi: 10.1016/S0898-8838(10)62008-9
  • High-Level Spectroscopy, Quantum Chemistry, and Catalysis: Not just a Passing Fad Frank Neese Angew. Chem. Int. Ed. (2017) 56, 11003 – 11010 doi: 10.1002/anie.201701163
• X-ray crystallography
  • An Introduction to X‐Ray Crystallography, Keith S Wilson (2018) doi: 10.1002/9780470015902.a0025432