[Expired] Joint PhD (Madrid-Grenoble): Structure-function studies of the nitrogenase iron-molybdenum cofactor (FeMo-co) maturase NifB

Joint PhD between Universidad Politecnica de Madrid (Dr Luis Rubio) and Université Grenoble-Alpes (Dr Yvain Nicolet) on the Structure-function studies of the nitrogenase iron-molybdenum cofactor (FeMo-co) maturase NifB. PhD training will include techniques in Molecular Biology, Synthetic Biology, anaerobic protein purification, protein crystallization and X-ray determination of protein structures. NifB is a Radical-SAM protein that carries out the unique reaction of producing a carbide atom and using it to fuse two [4Fe-4S] clusters into a complex structure that serves as precursor to FeMo-co. NifB has enormous fundamental and agronomic importance. This work is part of the project “Nitrogenase Biotechnological Applications” funded by MINECO (BIO2014-59131-R).